human r d systems sst2 elisa kit  (R&D Systems)

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Serum sST2 increased in CVDs. Serum sST2 levels in (A) men and women (black), (B) women only (peach), or (C) men only (blue) were compared between control patients without known CVDs (white) to those with myocarditis (Myo), cardiomyopathy (Card), coronary artery disease (CAD), myocardial infarct (MI), or congestive heart failure (CHF). Violin plots denote data distribution for each group with p -value calculated using Welch’s t -test: *, p < 0.05; ** , p < 0.01; *** , p < 0.001; **** , p < 0.0001; ns, not significant.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques: Control

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Sex differences in sST2 in controls and CVDs. sST2 levels compared women (peach) vs. men (blue) for (A) controls, (B) myocarditis (Myo), (C) cardiomyopathy (Card), (D) coronary artery disease (CAD), (E) myocardial infarction (MI), or (F) congestive heart failure (CHF). Violin plots denote data distribution for each group with p -value calculated using unpaired Student’s t -test: ** , p < 0.01; **** , p < 0.0001; ns, not significant.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Sex and age differences in sST2 in CVDs. sST2 levels compared women (peach) vs. men (blue) that were <50 vs. ≥50 years of age for (A) myocarditis (Myo), (B) cardiomyopathy (Card), (C) coronary artery disease (CAD), (D) myocardial infarction (MI), or (E) congestive heart failure (CHF). Violin plots denote data distribution for each group. One-way ANOVA: (A) p = 0.0043, (B) p = 0.035, (C) p = 0.94, (D) p = 0.16, and (E) p = 0.70 with two-way comparisons analyzed using unpaired Student’s t -test: *, p < 0.05; *** , p < 0.001; ns, not significant. (A) Two-way ANOVA p -values indicate no interaction between sex and age in patients with myo ( p = 0.11), but interactions between sex and sST2 ( p = 0.0047) and age and sST2 ( p = 0.0496). (B) Two-way ANOVA p -values indicate no interactions between sex and age ( p = 0.43) or age and sST2 ( p = 0.99) in patients with card, but interaction between sex and sST2 ( p = 0.04). (C) Two-way ANOVA p -values indicate no interactions between sex and age ( p = 0.60), age and sST2 ( p = 0.79), or sex and sST2 ( p = 0.79) in patients with CAD. (D) Two-way ANOVA p -values indicate no interactions between sex and age ( p = 0.32), age and sST2 ( p = 0.25), or sex and sST2 ( p = 0.92) in patients with MI. (E) Two-way ANOVA p -values indicate no interactions between sex and age ( p = 0.87), age and sST2 ( p = 0.54), or sex and sST2 ( p = 0.57) in patients with CHF.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Soluble ST2 (sST2) in pre- vs. post-menopausal women with CVDs. sST2 levels compared pre-menopausal women (light peach) to post-menopausal women (dark peach) for (A) cardiomyopathy (Card), (B) coronary artery disease (CAD), (C) myocardial infarction (MI), or (D) congestive heart failure (CHF). Violin plots denote data distribution for each group with p -value calculated using unpaired Student’s t -test: *, p < 0.05; ns, not significant.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Soluble ST2 (sST2) levels in women with early menopause. sST2 levels compared women with early menopause (Early, magenta) vs. those with normal menopause (peach) for (A) cardiomyopathy (Card), (B) coronary artery disease (CAD), (C) myocardial infarction (MI), or (D) congestive heart failure (CHF). Violin plots denote data distribution for each group with p -value calculated using unpaired Student’s t -test: *, p < 0.05; ns, not significant.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Sex differences in LVEF and sST2. sST2 levels compared women (peach) vs. men (blue) with a left ventricular ejection fraction (LVEF) of ≥45% vs. <45% for (A) myocarditis (Myo), (B) cardiomyopathy (Card), (C) coronary artery disease (CAD), (D) myocardial infarction (MI), or (E) congestive heart failure (CHF). Violin plots denote data distribution for each group. One-way ANOVA: (A) p = 0.024, (B) p = 0.023, (C) p = 0.79, (D) p = 0.17, and (E) p = 0.36 with two-way comparisons analyzed using unpaired Student’s t -test: *, p < 0.05; ns, not significant. (A) Two-way ANOVA p -values indicate no interaction between EF and sex ( p = 0.59) in patients with myocarditis, but interactions between LVEF and sST2 ( p = 0.04) and sex and sST2 ( p = 0.0036). (B) Two-way ANOVA p -values indicate no interactions between EF and sex ( p = 0.81) or EF and sST2 ( p = 0.22) in patients with cardiomyopathy, but interactions between sex and sST2 ( p = 0.01). (C) Two-way ANOVA p -values indicate no interactions between EF and sex ( p = 0.34), EF and sST2 ( p = 0.48), or sex and sST2 ( p = 0.40) in patients with CAD. (D) Two-way ANOVA p -values indicate no interactions between EF and sex ( p = 0.77), EF and sST2 ( p = 0.23), or sex and sST2 ( p = 0.39) in patients with MI. (E) Two-way ANOVA p -values indicate no interactions between EF and sex ( p = 0.21), EF and sST2 ( p = 0.87), or sex and sST2 ( p = 0.85) in patients with CHF.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Sex differences in LVEF and sST2 using ESC guidelines. sST2 levels compared women (peach) vs. men (blue) in patients with HFrEF, HFmrEF, or HFpEF for (A) myocarditis (Myo), (B) cardiomyopathy (Card), (C) coronary artery disease (CAD), (D) myocardial infarction (MI), or (E) congestive heart failure (CHF). Violin plots denote data distribution for each group. One-way ANOVA: (A) p = 0.053, (B) p = 0.032, (C) p = 0.72, (D) p = 0.093, and (E) p = 0.89 with two-way comparisons analyzed using unpaired Student’s t -test: *, p < 0.05; ns, not significant. (A) Two-way ANOVA p -values indicate no interaction between sex and ejection fraction (EF) in patients with myocarditis ( p = 0.51), or EF and sST2 ( p = 0.29), but interaction between and sex and sST2 ( p = 0.025). (B) Two-way ANOVA p -values indicate no interactions between sex and EF ( p = 0.69), EF and sST2 ( p = 0.16), or sex and sST2 ( p = 0.15) in patients with cardiomopathy. (C) Two-way ANOVA p -values indicate no interactions between sex and EF ( p = 0.89), EF and sST2 ( p = 0.20) or sex and sST2 ( p = 0.81) in patients with CAD. (D) Two-way ANOVA p -values indicate no interactions between sex and EF ( p = 0.42) and EF and sST2 ( p = 0.77), but interaction between sex and sST2 ( p = 0.032) in patients with MI. (E) Two-way ANOVA p -values indicate no interactions between sex and EF ( p = 0.95), EF and sST2 ( p = 0.68), or sex and sST2 ( p = 0.84) in patients with CHF.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Soluble ST2 (sST2) relationship to LVEF by sex. sST2 levels in men and women (black), women only (peach), or men only (blue) were correlated to % left ventricular ejection fraction (LVEF) for (A) myocarditis (Myo), (B) cardiomyopathy (Card), (C) coronary artery disease (CAD), (D) myocardial infarction (MI), or (E) congestive heart failure (CHF). P -values were calculated using 2-tailed Pearson correlation.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Sex differences in NYHA class and sST2 levels. sST2 levels compared women and men (gray), women (peach), or men (blue) to New York Heart Association (NYHA) class I–II vs. III–IV heart failure for (A) myocarditis (Myo), (B) cardiomyopathy (Card), (C) coronary artery disease (CAD), (D) myocardial infarction (MI), or (E) congestive heart failure (CHF). Violin plots denote data distribution for each group. One-way ANOVA: (A) p = 0.0002, (B) p = 0.66, (C) p = 0.64, (D) p = 0.55, and (E) p = 0.28. (A) Two-way ANOVA p -values indicate no interaction between NYHA Class and sex ( p = 0.59) in patients with myocarditis, but interactions between NYHA Class and sST2 ( p = 0.04) and sex and sST2 ( p = 0.0002). (B) Two-way ANOVA p -values indicate no interactions between NYHA Class and sex ( p = 0.94), NYHA Class and sST2 ( p = 0.74), or sex and sST2 ( p = 0.22) in patients with cardiomyopathy. (C) Two-way ANOVA p -values indicate no interactions between NYHA Class and sex ( p = 0.63), NYHA Class and sST2 ( p = 0.32), or sex and sST2 ( p = 0.52) in patients with CAD. (D) Two-way ANOVA p -values indicate no interactions between NYHA Class and sex ( p = 0.75), NYHA Class and sST2 ( p = 0.80), or sex and sST2 ( p = 0.20) in patients with MI. (E) Two-way ANOVA p -values indicate no interactions between NYHA Class and sex ( p = 0.12), NYHA Class and sST2 ( p = 0.72), or sex and sST2 ( p = 0.40) in patients with CHF. *, p < 0.05; **, p < 0.01.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques:

Journal: Frontiers in Cardiovascular Medicine

Article Title: Sex and age differences in sST2 in cardiovascular disease

doi: 10.3389/fcvm.2022.1073814

Figure Lengend Snippet: Soluble ST2 (sST2) relationship to NYHA class by sex. sST2 levels compared women (peach) vs. men (blue) with New York Heart Association (NYHA) class I–II vs. III–IV heart failure for (A) myocarditis (Myo), (B) cardiomyopathy (Card), (C) coronary artery disease (CAD), (D) myocardial infarction (MI), or (E) congestive heart failure (CHF). Data show simple linear regression of data for each group with 95% confidence intervals showing spread of certainty in the model. P -values were calculated using 2-tailed Pearson correlation.

Article Snippet: As this kit was not available when myocarditis samples were initially analyzed, the human R&D Systems sST2 ELISA kit (Cat# DST200, Minneapolis, MN, USA) was used for myocarditis samples.

Techniques: